殺蟲晶體蛋白和低聚異麥芽糖的毛細(xì)管電泳分析.pdf

1、華中農(nóng)業(yè)大學(xué)碩士學(xué)位論文殺蟲晶體蛋白和低聚異麥芽糖的毛細(xì)管電泳分析姓名：操麗麗申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：微生物學(xué)指導(dǎo)教師：喻子牛何進(jìn)20040603AbstractTheresearchresultsaresummarizedasfollowing：Purificationofcrystalproteins(95％)bypetroleumether—extractedandaqueoustwo—phaseextractionWaSdesc

2、ribedCrudeinsecticidalcrystalproteins(ICPs，133kDa)wasfirstdissolvedinNa2C030H105)，thenprecipitatedinHCl(1mol／L)andfinallypurifiedbyDEAEionexchangechromatographyAcapillaryzoneelectrophoresismethodandacapillarygelelectroph

3、oresisfordeterminingICPsinBacillusthuringiensisweredevelopedWith50mmol／Lborax，35mmol／LSDS，20％ACNandO1％pmercaptoethanolatpH95asrunningbufferICPscatlbedeterminedwithin10minattheappliedvoltageof10kVandthecapillarytemperatur

4、eof25“CThedimensionofcapillaryWas40cm75岫IDwithoutcoatingThelinearcorrelationcoefficientbetweentheconcentrationofICPsandthepeakareawasequalto09997intheconcentrationrangeof005～040medrrdTheexperimentconditionsofcapillarygel

5、electrophoresis：neutrallycoatedcapillary(50gmID，40cmoftotallengthand30cmofefficientlength)01mol／LTrisCHES(pH86)，01％SDS，25％PEOasrunningbuffer；theappliedvoltageof300V／cm；thecapillarytemperatureat25“Candthedetectionwave—len

6、gthWas214nlnAcapillaryeleetrophoresismethodforthedeterminationofmaincomponentsinisomaltooligosaccharideformulationwasdescribedTheinfluencesofvariOIlSseparartionconditionsincludingbufferconcentration，pHvalue，voltageanddet

7、ectionwave—lengthwereinvestigatedByusinganuncoatedsilicacapillary(75gmID，60cmoftotallength，and50cmofefficientlength)and75mmol／Lborax0H105)ascarrierelectrolyte，thederivatlzedisomaltooligosaccharideswitha—naphymethamine，in

8、cludingisomaltose，panose，isomaltotriose，wer@sufficientlyseparatedatthequalificationofanappliedvoltageof15kVandaUVdetectionwave1engthof214mTheresultsindicatethattherelativestandarddeviationsformigrationtimesandpeaksareawe

9、realllessthaIl05％and2O％respectivelyQuantificationbythepeakareamethodallowedreproducibledeterminationoftheanalytesattheconcentrationrangeof0005～1000mg／m1Thismethodischaracterizedbyitssensitivity，reproducibilityandlowcosts