羊胃腸線蟲苯并咪唑類抗藥性檢測及PCR-SSCP分析.pdf

1、內(nèi)蒙古農(nóng)業(yè)大學碩士學位論文羊胃腸線蟲苯并咪唑類抗藥性檢測及PCRSSCP分析姓名：郝成申請學位級別：碩士專業(yè)：預防獸醫(yī)學指導教師：楊曉野20070501StudyofDetectionofBenzimidazolesResistanceandPCRSSCPAnalysisinGastrointestinalNematodesofSmallDomesticRuminantsAbstractAnthelminticresistanceofg

2、astro—intestinalnematodesofsmalldomesticruminantswasasevereproblemthrouhouttheworld，whichhasnotyetbeenconcenedinChinaWiththeneedforoursheepandgoatsproductionandforcontrolofparasitesthestudywascarriedoutinthreeaspectsTher

3、esultswereasfollows：(1)Asuspected—resistantwormpopulationfromadistrictinNingxiawasselectedbyhatchedinThiabendazole(TBZ)s01ionTheinitialEDs0valuewas005799／mlbyegghatchassay(EllA)But，theED50valuerisedto0146蚓mlafteravitrose

4、lectionexperimentandexceedthecommendedcriticalvalueofWorldAssociationfortheAdvancementofVeterinaryParasitology(WAAVP)(2)Withthevitroselectedresistantisolatepopulationandthesencitiveonesfromthefieldasthecontrolgroup3，Amod

5、ifiedlarvaldevelopmentassay(LDA)wasusedasthemethodtodetectBenzimidazoles(BZs)，Levamisole(LEV)andAvermectins(A、，Ms)(3)Thepolymorphisminpartialfragmentofthebetatubulingenewasexaminedin且contortusfromtreatedanduntreatedpopul

6、ationsusingsingle—strandconfirmmionpolymorphism(SSCP)analysisTheresultsof181maleadultsanalysisrevealed20／(3／15)mutantindividuals60％(9／15)heterozygousonesand20％(3／15)normalonesinthetreatedpopulations，and36％(6／166)，78％(13／

7、166)and886％(147／166)respectivelyinthenaturalinfectiouspopulationsKeywords：Sheepandgoats；Anthelminticresistance；SelectionexperimentLarvaldevelopmentassay；PCRSSCPtechniqueDireotedby：ProfYangXiaoyeAppIicantforgasterDegree：H