初情期母牦牛下丘腦kiss-1-GPR54系統(tǒng)表達(dá)及激素分泌研究.pdf

1、Qinghai University Master Dissertation Study on Hypothalamic Expression of Kiss-1/GPR54 System and Hormones Secretion in Puberal female yak Supervisor: Jun Zhang Candidate: Zhiqiang Wang Academic Major Applided for: Basi

2、c Veterinary Medicine Specific Major: Plateau animal physiology College (Department): Academy of agricµ lture and animal Husbandry April, 2017縮略詞表 II Abstract The study in order to explore the puberty activate char

3、acteristic of puberal female yak, we choose puberal female yak as research object, enzyme linked immunosorbent assay (Elisa) was performed to measured the concentration of GnRH、LH、FSH in puberal female yaks during oestru

4、m 24 hours; Using he matoxyin-eosinstaining (HE) to dye ovary、uterus and breast tissue and observed compare each tissues growth and development situation; biology technique was performed to determine CDS sequence of two

5、influence mammal oestrus genes:kiss-1 gene and GPR54 gene and analysis homology. hypothalamic kiss-1 mRNA and GPR54 mRNA in estrus female yak and anestrus female yak were analyzed by Real-time PCR, next immunohistochemic

6、al methods were performed to measured the expression of kisspeptin nuclzi in the hypothalamic of estrus female yak and anestrus female. Our research result were as follow: 1.during oestrum, estrus female yak’s blood GnRH

7、 、 LH and FSH concentration were higher than anestrus female yak, the secretion of GnRH and LH were positive correlation. 2.estrus female yak’s ovary include primary follicle and secondary follicle, anestrus female yak’s

8、 ovary include primary follicle and atretic follicle. hematoxyin-eosinstaining dye for uterus was that estrus female yak’s endometrium contain a large number of shallow gland and deep gland, it’s more than anestrus femal

9、e yak, but enlarge gland was less than anestrus female yak (P>0.05); HE dye for breast reveal ,estrus female and anestrus female yak’s mammary gland epithelial were simple cuboidal epithelium, estrus female yak breast

10、’s interlobar duct,acini was developer than anestrus female yak. 3.Puberal female yak’s kiss-1 gene and GPR54 gene CDS sequence were determined. The result of homology analysis show: yak’s kiss-1 gene sequence、GPR54 gene