轉(zhuǎn)基因三倍體毛白楊的抗蟲基因表達水平及抗蟲機理研究.pdf

1、河北農(nóng)業(yè)大學(xué)碩士學(xué)位論文轉(zhuǎn)基因三倍體毛白楊的抗蟲基因表達水平及抗蟲機理研究姓名：袁勝亮申請學(xué)位級別：碩士專業(yè)：森林保護指導(dǎo)教師：高寶嘉楊敏生20050606Studyontheinsect—resistancegeneexpressionlevelandmechanismoftransgenictriploidChinesewhitepopularMastercandidate：：YUANsheng—liangSpeciality：Fo

2、restprotectionAdviser：GAOBaojiaprofessorYANGMin—shengprofessorabstractThestudyisaimedtoscreenthevariesinsectresistanceclonesofthetriploidofChinesewhitepoplarthroughfeedingLymentriadispa21(Linnaeus)andClosteraanachoreta(F

3、abricius)test，andtovalidatetheinsect—resistancecharacterthroughthetestofmoleculebiologydetection，theruleoftheexpressionofextrinsicgene，thepersistenceofinsect—resistance，thedetoxifcationenzymeanddigestiveenzymeoftheinsect

4、sandmicroobservationofthemidgut(1)TherearenulesintheexpressionchangesoftheinsectresistanceofthetriploidofChinesewhitepopularTheinsectswerefedon29clonesofthetriploidofChinesepopularThereare13cloneshavingthehigherinsectres

5、istanceandaccountfor4483％ofalltheclonesThemortatityofClosteraanachoreta(Fabricius)fedonthehighinsectresistancepopularisamong80％and100％Therearc10cloneshavingthemiddleinsect—resistanceandaccountfor3448％ofalltheclonesThemor

6、talityofClosteraanachoreta(Fabricius)fedonthe10clonesisamong60％and79％Thereare6cloneshavingthelowerinsectresistanceandaccountfor2069％ofalltheclones，thedeathrateofClosteraanachoreta(Fabriciuslfedonthoseclonesisamong145％and

7、50％Comparedwithfeedingtestresultsof2003thereare8clonesofinsectresistancechangedThe2and10cloneswhichwerehigherresistancein2003，changedtomiddleinsectresistancein2004Themiddleinsectresistance，45，46，and47clonesin2003changedl

8、ohigherinsectresistancein2004Thelowerresistance19cloneschangedtohighinsectresistance，24and28cloneschangedtomiddleinsectresistanceclonesin2004(2)TransgenictriploidChinesewhitepoplarhaslastingresistingworm。seffectThe3and4e

9、raofCtosteraanachoreta(Fabricius)arefedonthehigherinsectresistance1and7clones，themiddleinsect—resistance10and28clonesandthelowerinsectresistance26and16clonesrespectivelyThedeathrateofthe4thgenerationfedonthehigherandmidd

10、leinsectresistanceclonesand26clonesishigherthanthe3rdgenerationThemothweightofthe3rdgenerationislowerthanthemothfedonCKThedeathrateofthe4thgenerationfedonthehigherandmiddleinsectresistanceclonesis100％Moreoverthedeathrate

11、ofthe4thgenerationfeededon26clonesishigherthantiledeathrateofthe3rdgenerationTheamountoflayingof3rdgenerationfedon1and7clonesislargelysuppressedAndtheeffecttolarvaefedon10and28clonesislighterthanfedoiltheIand7clones(3)Th

12、einfluenceoftransgenictriploidChinesewhitepoplartothedetoxificationenzymesandmidgutdigestiveenzymesTheactivitiesofesterase，carboxylesteraseandmix—functionoxidaseoftheCtosteraanachoreta(Fabricius)larvaeareinverseratiowith